Part:BBa_K4806216:Design

CYPCamC gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1023
  Illegal PstI site found at 1295
  Illegal PstI site found at 2019
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal PstI site found at 1023
  Illegal PstI site found at 1295
  Illegal PstI site found at 2019
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1023
  Illegal PstI site found at 1295
  Illegal PstI site found at 2019
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1023
  Illegal PstI site found at 1295
  Illegal PstI site found at 2019
  Illegal NgoMIV site found at 3040
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
• 2. CYPCamC gene (B3-B4) (BBa_K4806002)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.